Kpakpando n'ime sel stem! Nnyocha ụlọọgwụ na itinye sel eriri afọ mesenchymal stem

N'ime afọ ndị na-adịbeghị anya, nyocha nke mkpụrụ ndụ sel mesenchymal na-aga n'ihu na-abawanye, ma ka ọ dị ugbu a, eweghachila ihe karịrị 47000 akụkọ metụtara mkpụrụ ndụ mesenchymal sel na Pubmed.

Ndị ọkà mmụta sayensị na-ewere sel eriri afọ mesenchymal dị ka akụ ma na-egosipụtakwa ike ha nwere maka ọgwụgwọ ọrịa na mgbochi ịka nká.

Taa, onye nchịkọta akụkọ ga-akọwara gị ihe umbilical cord mesenchymal stem cell bụ, ma gosipụta mma nke eriri nwa mesenchymal stem cell na ngalaba ahụike site n'iwebata ngwa nyocha ụlọ ọgwụ nke ụbụrụ mesenchymal stem cell.

Gịnị bụ eriri nwa mesenchymal stem cell?

Umbical cord mesenchymal stem cell (hUC MSCs) na-esi n'ụdọ mmiri nke ụmụ amụrụ ọhụrụ na-adịkwa nchebe ma bụrụ nke ochie ma e jiri ya tụnyere sel ndị okenye mesenchymal. Ha na-enwe mmụba siri ike karị, dị iche iche, na ikike nchịkwa nchịkwa; Na mgbakwunye, e jiri ya tụnyere isi mmalite ndị ọzọ nke mkpụrụ ndụ sel mesenchymal, sel eriri afọ mesenchymal na-enweta site n'anụ ahụ dị iche iche na-enweghị mmerụ ọ bụla n'ahụ mmadụ. Ya mere, eriri afọ MSC kwesịrị ekwesị maka nyocha ụlọ ọgwụ na ngwa ngwa, ma bụrụ nhọrọ kachasị mma maka ọgwụgwọ cell na ọgwụ na-emegharị ahụ.

Umbilical cord mesenchymal stem cell egosila ike dị iche iche na vitro, gụnyere chondrocytes, adipocytes, osteoblasts, skeletal cell, cardiomyocytes, imeju mkpụrụ ndụ, yana imepụta glucagon cell, somatostatin secreting cell, glial cell (oligodendrocytes), na dopaminergic neurons. Ya mere, sel eriri afọ mesenchymal dị mma maka ngwa ụlọ ọgwụ na nyocha sayensị.

Mkpụrụ ndụ mesenchymal aghọọla ebe nyocha

N'afọ ndị na-adịbeghị anya, nyocha nke mkpụrụ ndụ mesenchymal na-arị elu, na ClinicalTrials sitere na NIH na United States bụ nchekwa data nyocha ụlọ ọgwụ kacha ukwuu n'ụwa. Dịka nsonaazụ ọchụchọ nke nchekwa data siri dị, dịka nke Mee 13, 2019, enwere ọmụmụ ụlọ ọgwụ 753 edebara aha na nchekwa data gbasara mkpụrụ ndụ mesenchymal. N'ime ha, ọnụ ọgụgụ ọmụmụ ụlọ ọgwụ emere na China, Europe, na United States so na atọ kachasị elu n'ụwa.

Site n'echiche nke ụdị ọrịa a na-agwọ, nchọpụta ụlọ ọgwụ na mkpụrụ ndụ mesenchymal na-agụnye ọtụtụ narị ọrịa. N'ime ha, ọrịa akwara ozi, ọrịa obi, na orthopedic bụ akụkụ atọ bụ isi nyocha, na-aza ihe karịrị 15%, na ngụkọta karịrị ọkara. Na mgbakwunye, oke nke ọrịa shuga, imeju, ngụgụ, eriri afọ, akpụkpọ ahụ, ọrịa autoimmune na graft versus host disease (GVHD) bụ ihe dịka 5%, nke bụ ntụzịaka nyocha ụlọ ọgwụ dị mkpa nke mkpụrụ ndụ sel mesenchymal.

Nyocha nyocha ngwa ụlọ ọgwụ nke eriri nwa mesenchymal stem cell

01.ọrịa ndị na-eme nri

1.1 Ọrịa shuga mellitus

Ka ọ dị ugbu a, enweghị ọgwụgwọ maka ọrịa shuga n'ụlọ ọgwụ, anyị nwere ike ịdabere naanị na insulin ma ọ bụ ọgwụ in vitro iji belata shuga ọbara, ma n'otu oge ahụ na-akwado nri na mmega ahụ. Selụ sel mesenchymal nwere ikike ịkpali agwaetiti pancreatic β Ọrụ mmụgharị cell.

Na China, enwere ụlọọgwụ atọ kachasị elu nke mere nyocha ụlọ ọgwụ gbasara ntụgharị mkpụrụ ndụ mesenchymal na ọgwụgwọ ụdị ọrịa shuga II. Ọtụtụ ndị ọrịa enwetala mmetụta nke ibelata shuga ọbara na insulin, ụfọdụ na-akwụsịkwa ịṅụ ọgwụ. N'otu oge ahụ, ọnọdụ anụ ahụ nke onye ọrịa akawanyela mma ma dozie ọnọdụ ahụike ha ruo n'ókè ụfọdụ.

Ụlọ ọgwụ Fuzhou General nke Nanjing Military Region nyochara nchekwa na ịdị irè nke eriri afọ mesenchymal stem cell jikọtara na autologous ọkpụkpụ ọkpụkpụ mesenchymal sel na ọgwụgwọ nke ụdị ọrịa shuga M na-enweghị immunotherapy. Nsonaazụ gosiri na mmetụta ọgwụgwọ nke ndị ọrịa 42 dị mma. C-Pep mụbara site na 105.7%, insulin 49.3%, HbA1c belatara site na 12.6%, glucose ọbara ngwa ngwa belatara site na 24.4%, mkpa insulin belatara site na 29.2%.

1.2 Nsogbu nke ọrịa shuga

Mesenchymal stem cell nwere atụmanya itinye aka na nsogbu nke ọrịa shuga.

1.2.1 Ụkwụ ọrịa shuga

N'ụlọọgwụ Shaanxi Provincial People's Hospital, n'okpuru ọnọdụ adịghị mma, a na-agbanye eriri nwa mesenchymal stem cell nkwusioru n'ime akwara n'oge 3cm x 3cm na ọnya nke ndị ọrịa 5 nwere ụdị ọrịa shuga II nke nwere ụkwụ na-arịa ọrịa shuga gbagwojuru anya. Maka ndị nwere ọnya siri ike, a na-ekewa injections 1cm x 1cm n'ụkwụ, na-elekwasị anya na ịgbanye n'akụkụ ọnya ahụ. Mgbe a gwọchara ya, ọ̀tụ̀tụ̀ mgbu ụkwụ na ahụ́ erughị ala na-ebelata nke ukwuu, claudication na-adịte aka na-akawanye mma nke ọma, ọnya ọnya gwọkwara kpamkpam.

N'ụlọọgwụ Anhui Provincial nke Mahadum Ahụike Anhui, a gbabanyere eriri nwa mesenchymal stem cell n'ime ebe ọnya akụkụ akụkụ ahụ metụtara nke ndị ọrịa 53 nwere ọkwa II-IV nwere ụkwụ nwere ọrịa shuga. Mgbe ọnwa atọ nke ọgwụgwọ gasịrị, ma e jiri ya tụnyere ndị na-ahụ maka njikwa ahụ, otu ọgwụgwọ ahụ gosipụtara ọganihu dị ukwuu na okpomọkụ akpụkpọ anụ, nrịanrịa nrụgide nkwonkwo ụkwụ-brachial, esemokwu oxygen akpụkpọ anụ, na ebe dị anya.

1.2.2 Ọrịa mamịrị nephropathy

Ụlọ akwụkwọ ahụike ahụike nke Fuzhou General Hospital, Mahadum Ahụike nke Abụọ, na Ụlọ Ọgwụ Changzheng nke Abụọ nke Mahadum Ọgwụ nke Abụọ na-agwọ ndị ọrịa 15 nwere eriri afọ mesenchymal stem cell site na ntinye aka n'ime akwara azụ azụ nke pancreatic, akwara akwara abụọ, na infusion vein peripheral. . Mgbe ọgwụgwọ gasịrị, ndị ọrịa ahụ gosipụtara mmụba nke ọbara mgbali elu, shuga ọbara, ọkwa C-peptide, na ihe ngosi ọrụ gbasara akụrụ. N'ihe banyere ịdị irè nke ụlọ ọgwụ n'ịkwalite ọbara mgbali elu diastolic na ọrụ gbasara akụrụ, otu ọgwụgwọ ahụ karịrị ndị otu njikwa ejiri telmisartan ọnụ gbakwunyere insulin subcutaneous.

02.Ọgba aghara akwara

2.1 Ọrịa Parkinson

Akpa ikpe nke mbugharị cell stem maka ọrịa Parkinson na China ka emechara n'ụlọọgwụ dị na Shijiazhuang. Xie Xiyun, onye dị afọ 76 si Indonesia, nọ na-arịa ọrịa Parkinson kemgbe ihe karịrị afọ iri, na-enwe isi ike na mgbakasị ahụ n'ime mkpịsị aka atọ nke aka nri ya, ahụ siri ike, ihe isi ike ilo, na enweghị ike ilekọta onwe ya. N'ime awa ole e nwetara ngbanwe cell nke mesenchymal, ọ nwere ike rie nri nke ọma ma na-ekiri telivishọn na mgbede ahụ, na-enwe ọganihu dị ukwuu na mgbaàmà ndị ọzọ.

Dr. Sun Li si China Medical University webatara nkwusioru nwere eriri afọ mesenchymal stem cell n'ime akwara olu nke ndị ọrịa 10 Parkinson. Mgbe a tụgharịrị ya, ndị ọrịa ahụ gosipụtara mmelite n'ọkwa ije, oke ịma jijiji, mmegharị afọ ofufo, na ikike ịnọdụ ala na iguzo ọtọ. Ọ bụghị naanị na ọ na-achịkwa ọganihu nke ọrịa Parkinson nke ọma, kamakwa ọ na-eme ka ọrụ ụbụrụ ụbụrụ mebiri emebi dịkwuo mma, si otú ahụ na-eme ka ndụ ndị ọrịa dịkwuo mma.

2.2 senile dementia

Ndị otu Zhou Qiang duziri n'ụlọọgwụ Jinyang dị na Guiyang City ji mkpụrụ ndụ ụmị ọkpụkpụ na-akpachapụ anya gwọọ ndị ọrịa nkwarụ anọ. Onye ọrịa nke mbụ, nwoke dị afọ 75, amataghị ndị ezin’ụlọ mgbe a nabata ya ma nwekwaghị eriri afọ na njikwa afọ. N'ịgbaso ọgwụgwọ mbugharị, mgbaàmà ndị a na-akawanye ngwa ngwa, na mgbe ọ gbasasịrị, ọ bụghị nanị na ọ ghọtara ndị ezinụlọ ya kamakwa ọ nwetaghachiri eriri afọ na ọrụ afọ.

2.3 ehighị ụra nke ọma

Dr. Wang Yali si n'ụlọ ọgwụ ndị uwe ojii Zhejiang na-agwọ ndị ọrịa 19 nwere ehighị ụra nke ọma site na ntinye nke eriri afọ nke mkpụrụ ndụ mesenchymal. Ndị ọrịa a niile nwetara ihe isi ike ihi ụra, ebelata ogo ihi ụra, yana ngụkọta oge ụra nke na-erughị awa 6. Mgbe otu ọnwa ọgwụgwọ gasịrị, mmụba nke ogo ihi ụra nke ndị ọrịa dị ka nke ọgwụ ọnụ. Mgbe ọnwa abụọ nke ọgwụgwọ gasịrị, ọnụọgụ ụra nke ndị ọrịa dị mma karịa nke ọgwụ ọnụ. Mgbe ọnwa atọ nke ọgwụgwọ gasịrị, ịdị mma ụra nke ndị ọrịa dịkwa mma karịa nke ọgwụ ọnụ, na mmetụta nke otu ọgwụgwọ nwere ike ịdịru ihe ruru otu afọ, na-eme ka ogo ihi ụra dịkwuo mma na ndị ọrịa.

03.Mgbasa akwara

3.1 Ọrịa nkwonkwo degenerative

N'afọ ndị 1990, Brittberg na ndị ọzọ malitere iji nweta nsonaazụ ụfọdụ n'imezi mmerụ ahụ nke cartilage site na iji chondrocytes autologous. Ya mere, ọmụmụ ihe na-aga n'ihu malitere ilekwasị anya na mkpụrụ ndụ mesenchymal. Mesenchymal stem cell nwere ike ịwapụta n'ime anụ ahụ dị iche iche ma rụkwaa cartilage elu articular na ọkpụkpụ subchondral.

N'ime nyocha nke Akgun et al. site na Ngalaba Orthopedics na Traumatology na Mahadum Istanbul dị na Turkey, ha tụnyere ọgwụgwọ ọgwụgwọ nke autologous marrow mesenchymal stem cell na chondrocytes na ndị ọrịa 14 nwere ọrịa ogbu na nkwonkwo. Ha chọpụtara na ndị ọrịa na-anata ọgwụgwọ sel sel mesenchymal gosipụtara mmụba dị ịrịba ama na mmerụ ikpere ikpere na akara nsonaazụ osteoarthritis (KOOS) na akara analọgụ visual (VAS).

Wakitani et al. site na Feinberg School of Medicine na Northwestern University na Cleveland Clinic Hospital na Ohio University mere ule ụlọ ọgwụ site na iji mkpụrụ ndụ mesenchymal. N'ime otu nnwale ahụ, a na-etinye gel collagen jikọtara ya na ụmị ọkpụkpụ nke autologous mesenchymal stem cell na-etinye n'ime ọnyá ndị ọrịa articular cartilage, ebe ndị na-ahụ maka njikwa na-enweta nanị nha nha nke gel collagen. Otu abụọ ahụ gosipụtara ọganihu na ọrụ nkwonkwo, ma ọ bụ naanị otu nnwale ahụ gosipụtara ịmaliteghachi cartilage transperent na saịtị nke mmerụ ahụ. Ha sochiri ndị ọrịa 41 natara ọgwụgwọ mkpụrụ ndụ mesenchymal ruo afọ iri na otu na-ahụtaghị nsogbu siri ike.

04.ọrịa usoro ọmụmụ

4.1 Amụghị nwa

Imezi mmebi siri ike na endometrium, nke bụ nrọ nke ọtụtụ ụmụ nwanyị na-amụghị nwa, na-ekwe omume ugbu a site na teknụzụ mesenchymal stem cell regenerative, na-eweta olileanya imezu nrọ ụmụ nwanyị banyere ịbụ nne.

Ndị otu Prọfesọ Hu Yali sitere na ngalaba Obstetrics na Gynecology nke ụlọ ọgwụ Gulou dị na Nanjing, na-arụkọ ọrụ na ndị otu Prọfesọ Dai Jianwu sitere na Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, mepụtara nke ọma, maka oge mbụ mba ụwa, usoro iji jikọta collagen scaffolds na mesenchymal stem cell. Site na ijikọ usoro hysteroscopic ọdịnala, ha nwetara nrụzi arụmọrụ nke endometrium mebiri emebi, na-enyere ndị ọrịa atọ aka imezu nrọ ha nke ịghọ nne.

Nwada Hu si Yancheng, Jiangsu, nwetara ime ọpụpụ atọ sitere n'okike n'etiti afọ 31 na 34, na-eduga na nrapanye siri ike n'ime oghere uterine ya n'ihi ịwa ahụ nke uterine mgbe ime ọpụpụ gasịrị. N'iji mkpụrụ ndụ mesenchymal mesenchymal, ụlọ ọgwụ Gulou dị na Nanjing rụziri nke ọma endometrium ya n'ime ọnwa asatọ. Nke a mere ka o nwee ike ịtụrụ ime nke ọma, na July 17, 2014, ọ mụrụ "nwa ọhụrụ ọgwụ na-emegharị ahụ" na China.

Nanjing Gulou Hospital's mesenchymal stem cell "endometrial regeneration surgery" emela ka ndị ọrịa 13 na-amụghị nwa nwere nnukwu oghere uterine nwere ike ịtụrụ ime wee mụọ nwa, na-ebute ngụkọta nke 14 ahụike "ụmụ ọhụrụ ọgwụ na-emegharị ahụ."

4.2 Ovarian ọdịda

Ọdịda ovarian akaghi aka na-ezo aka n'ihe nke ụmụ nwanyị na-enweta atrophy ovarian ma kwụsị ịkwa akwa tupu ha eruo afọ 40. Ugbu a, ihe dịka 1% nke ụmụ nwanyị na-amụ nwa n'ụwa nile na-enweta ọdịda ovarian akaghi aka, na-eme ka ọ bụrụ nnukwu ihe na-akpata amụghị nwa.

Prọfesọ Wu Ji na ndị otu ya sitere na Mahadum Shanghai Jiao Tong dị na China kewapụrụ sel ụmụ nwanyị na-amụ nwa site na ụmụ oke transgenic protein fluorescent ndụ ndụ dị otu izu ruo izu abụọ. Mgbe emechara ọcha, omenala na usoro ndị ọzọ, a tụgharịrị mkpụrụ ndụ ndị a n'ime ụmụ oke nwere ọdịda ovarian akaghi aka. Nsonaazụ nyocha ahụ gosiri na mkpụrụ ndụ sel ụmụ nwanyị na-amụ nwa, dị ka mkpụrụ ndụ stem ndị ọzọ, nwere ihe ndị na-emepụta ihe. Mgbe e mesịrị, ụfọdụ mkpụrụ ndụ na-ekewa ma jiri nwayọọ nwayọọ ghọọ oocytes tozuru okè, si otú ahụ na-eweghachi ọrụ ovarian.

4.3 Arụ ọrụ erectile (ED)

Iji usoro ọgwụgwọ sel steepụ maka arụrụ arụ erectile nwere ike dochie sel anụ ahụ mebiri emebi ma ọ bụ nwụrụ anwụ wee zoo ihe iji rụkwaa mkpụrụ ndụ anụ ahụ penile na-arụ ọrụ nke ọma.

Ndị ọrịa iri na ise na-arụ ọrụ erectile na Denmark nwetara infusions cell mesenchymal na-enweghị ọgwụ ma ọ bụ ntụgharị penile. Mgbe ọnwa isii gachara, ụmụ nwoke asatọ nwere ike inwe mmekọahụ nke ọma.

05.Ọrịa na-egbu onwe ya

N'afọ 2007, Prọfesọ Sun Lingyun na China ji mkpụrụ ndụ mesenchymal mesenchymal mesoo okwu abụọ nke lupus erythematosus na-emeghachi omume nke ọma, n'afọ sokwa ya, ọ gwọkwara ọrịa itoolu ọzọ. Mgbe ọgwụgwọ ahụ gasịrị, a na-ebelata mgbaàmà nke ndị ọrịa ahụ nke ọma, ọ dịghịkwa nlọghachi ọzọ n'oge ntinye ọnwa 6.

N'afọ 2012, Ngalaba Rheumatology na Immunology nke Ụlọ Ọgwụ Jiangsu University Affiliated Hospital na-etinye n'ime eriri afọ mesenchymal stem cell maka ndị ọrịa 21 nwere lupus erythematosus systemic, nke na-ebelata ọkwa nke protein urinary n'ime ndị ọrịa a, belata ọrụ ọrịa, ma wedata Systemic Lupus Erythematosus. Akara Arụ Ọrụ Ọrịa.

Mmetụta nke ntụgharị mkpụrụ ndụ mesenchymal na ọrụ ọrịa yana protein urinary na ndị ọrịa nwere lupus erythematosus (SLE)

N'afọ 2013, Ngalaba Rheumatology na Immunology nke Ụlọ Ọgwụ Mbụ nke Mahadum Yangtze na Jingzhou First People's Hospital na-elekọta cell eriri afọ mesenchymal site na vein ihu cubital nye ndị ọrịa 18 nwere usoro lupus erythematosus. Otu ọnwa ka e mesịrị, ọtụtụ ndị ọrịa gosipụtara ọganihu dị ukwuu na mgbaàmà ụlọ ọgwụ na ihe ịrịba ama dị ka ahụ ọkụ, ike ọgwụgwụ, na adịghị ike ma e jiri ya tụnyere tupu a na-atụgharị ya.

06. mgbochi ịka nká

Ihe metụtara ịka nká nke ahụ mmadụ na ọnụọgụ cell ezughị ezu ma ọ bụ adịghị arụ ọrụ. Ka afọ na-abawanye, a na-enwe mgbanwe na-agbadata ma ọnụọgụ na ịdịmma nke sel sel n'ime ahụ. Omume a na-eduga n'ọdịda na ịka nká nke mkpụrụ ndụ mebiri emebi yana akụkụ ahụ na anụ ahụ mejupụtara sel, na-ebute ahụike na ịka nká nke ọma. Mesenchymal stem cell nwere ike inwe ọtụtụ ike dị iche iche, nke nwere ike ịwapụta n'ime ụdị sel stem dị iche iche dị mkpa n'ime ahụ. Site n'ịgbakwụnye mkpụrụ ndụ sel ezughị ezu n'ime ahụ na ịrụzi akụkụ ahụ na anụ ahụ mebiri emebi, mkpụrụ ndụ sel mesenchymal na-ekere òkè na mgbochi ịka nká.

Site na mmụba mmasị na ntinye nke nyocha mkpụrụ ndụ mesenchymal stem cell, a kwenyere na n'ọdịnihu dị nso, a ga-etinye ha n'ọtụtụ ebe na ntọala ụlọ ọgwụ, si otú ahụ na-enye uru dị ukwuu maka ahụike mmadụ.